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human prorenin enzyme  (Innovative Research Inc)


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    Innovative Research Inc human prorenin enzyme
    Effect of aliskiren on the cell-surface expression of full-length (P)RR. (A) Percentage of <t>prorenin</t> bound to the HUVEC membrane. The concentration of unbound prorenin in Medium-2 was determined <t>by</t> <t>ELISA,</t> and the bound prorenin was determined as described in the Materials and methods section. (B) Percentage of bound prorenin per HUVEC. The percentage of bound prorenin was divided by the cell number. (C) Renin activity per cell for aliskiren-treated cells and controls. The concentration of AngI in Medium-3 was determined by ELISA to calculate renin activity. Renin activity was divided by the cell number. Filled squares represent aliskiren-treated cells and open circles represent controls. Statistical differences were identified between aliskiren-treated cells and controls by a two-tailed unpaired Student's t-test with P<0.05 as the limit of significance. HUVEC, human umbilical vein endothelial cell; AngI, angiotensin I; (P)RR, (pro)renin receptor.
    Human Prorenin Enzyme, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human prorenin enzyme/product/Innovative Research Inc
    Average 90 stars, based on 15 article reviews
    human prorenin enzyme - by Bioz Stars, 2026-02
    90/100 stars

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    1) Product Images from "Aliskiren reduces the release of soluble (pro)renin receptor from human umbilical vein endothelial cells"

    Article Title: Aliskiren reduces the release of soluble (pro)renin receptor from human umbilical vein endothelial cells

    Journal: Biomedical Reports

    doi: 10.3892/br.2018.1124

    Effect of aliskiren on the cell-surface expression of full-length (P)RR. (A) Percentage of prorenin bound to the HUVEC membrane. The concentration of unbound prorenin in Medium-2 was determined by ELISA, and the bound prorenin was determined as described in the Materials and methods section. (B) Percentage of bound prorenin per HUVEC. The percentage of bound prorenin was divided by the cell number. (C) Renin activity per cell for aliskiren-treated cells and controls. The concentration of AngI in Medium-3 was determined by ELISA to calculate renin activity. Renin activity was divided by the cell number. Filled squares represent aliskiren-treated cells and open circles represent controls. Statistical differences were identified between aliskiren-treated cells and controls by a two-tailed unpaired Student's t-test with P<0.05 as the limit of significance. HUVEC, human umbilical vein endothelial cell; AngI, angiotensin I; (P)RR, (pro)renin receptor.
    Figure Legend Snippet: Effect of aliskiren on the cell-surface expression of full-length (P)RR. (A) Percentage of prorenin bound to the HUVEC membrane. The concentration of unbound prorenin in Medium-2 was determined by ELISA, and the bound prorenin was determined as described in the Materials and methods section. (B) Percentage of bound prorenin per HUVEC. The percentage of bound prorenin was divided by the cell number. (C) Renin activity per cell for aliskiren-treated cells and controls. The concentration of AngI in Medium-3 was determined by ELISA to calculate renin activity. Renin activity was divided by the cell number. Filled squares represent aliskiren-treated cells and open circles represent controls. Statistical differences were identified between aliskiren-treated cells and controls by a two-tailed unpaired Student's t-test with P<0.05 as the limit of significance. HUVEC, human umbilical vein endothelial cell; AngI, angiotensin I; (P)RR, (pro)renin receptor.

    Techniques Used: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Two Tailed Test

    Effect of aliskiren on the level of s(P)RR released from HUVECs. (A) Concentration of released s(P)RR. The concentration of s(P)RR in Medium-1 was determined by sandwich ELISA. (B) s(P)RR concentration per HUVEC cell. s(P)RR concentration was divided by the cell number. Filled squares represent aliskiren-treated cells and open circles represent controls. Statistical differences were identified between aliskiren-treated cells and controls by a two-tailed unpaired Student's t-test with P<0.05 as the limit of significance. s(P)RR, soluble (pro)renin receptor; HUVEC, human umbilical vein endothelial cell.
    Figure Legend Snippet: Effect of aliskiren on the level of s(P)RR released from HUVECs. (A) Concentration of released s(P)RR. The concentration of s(P)RR in Medium-1 was determined by sandwich ELISA. (B) s(P)RR concentration per HUVEC cell. s(P)RR concentration was divided by the cell number. Filled squares represent aliskiren-treated cells and open circles represent controls. Statistical differences were identified between aliskiren-treated cells and controls by a two-tailed unpaired Student's t-test with P<0.05 as the limit of significance. s(P)RR, soluble (pro)renin receptor; HUVEC, human umbilical vein endothelial cell.

    Techniques Used: Concentration Assay, Sandwich ELISA, Two Tailed Test



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    Innovative Research Inc human prorenin enzyme
    Effect of aliskiren on the cell-surface expression of full-length (P)RR. (A) Percentage of <t>prorenin</t> bound to the HUVEC membrane. The concentration of unbound prorenin in Medium-2 was determined <t>by</t> <t>ELISA,</t> and the bound prorenin was determined as described in the Materials and methods section. (B) Percentage of bound prorenin per HUVEC. The percentage of bound prorenin was divided by the cell number. (C) Renin activity per cell for aliskiren-treated cells and controls. The concentration of AngI in Medium-3 was determined by ELISA to calculate renin activity. Renin activity was divided by the cell number. Filled squares represent aliskiren-treated cells and open circles represent controls. Statistical differences were identified between aliskiren-treated cells and controls by a two-tailed unpaired Student's t-test with P<0.05 as the limit of significance. HUVEC, human umbilical vein endothelial cell; AngI, angiotensin I; (P)RR, (pro)renin receptor.
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    human prorenin enzyme-linked immunosorbent assay (elisa) kit  (Innovative Research Inc)
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    Effect of aliskiren on the cell-surface expression of full-length (P)RR. (A) Percentage of <t>prorenin</t> bound to the HUVEC membrane. The concentration of unbound prorenin in Medium-2 was determined <t>by</t> <t>ELISA,</t> and the bound prorenin was determined as described in the Materials and methods section. (B) Percentage of bound prorenin per HUVEC. The percentage of bound prorenin was divided by the cell number. (C) Renin activity per cell for aliskiren-treated cells and controls. The concentration of AngI in Medium-3 was determined by ELISA to calculate renin activity. Renin activity was divided by the cell number. Filled squares represent aliskiren-treated cells and open circles represent controls. Statistical differences were identified between aliskiren-treated cells and controls by a two-tailed unpaired Student's t-test with P<0.05 as the limit of significance. HUVEC, human umbilical vein endothelial cell; AngI, angiotensin I; (P)RR, (pro)renin receptor.
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    Effect of aliskiren on the cell-surface expression of full-length (P)RR. (A) Percentage of prorenin bound to the HUVEC membrane. The concentration of unbound prorenin in Medium-2 was determined by ELISA, and the bound prorenin was determined as described in the Materials and methods section. (B) Percentage of bound prorenin per HUVEC. The percentage of bound prorenin was divided by the cell number. (C) Renin activity per cell for aliskiren-treated cells and controls. The concentration of AngI in Medium-3 was determined by ELISA to calculate renin activity. Renin activity was divided by the cell number. Filled squares represent aliskiren-treated cells and open circles represent controls. Statistical differences were identified between aliskiren-treated cells and controls by a two-tailed unpaired Student's t-test with P<0.05 as the limit of significance. HUVEC, human umbilical vein endothelial cell; AngI, angiotensin I; (P)RR, (pro)renin receptor.

    Journal: Biomedical Reports

    Article Title: Aliskiren reduces the release of soluble (pro)renin receptor from human umbilical vein endothelial cells

    doi: 10.3892/br.2018.1124

    Figure Lengend Snippet: Effect of aliskiren on the cell-surface expression of full-length (P)RR. (A) Percentage of prorenin bound to the HUVEC membrane. The concentration of unbound prorenin in Medium-2 was determined by ELISA, and the bound prorenin was determined as described in the Materials and methods section. (B) Percentage of bound prorenin per HUVEC. The percentage of bound prorenin was divided by the cell number. (C) Renin activity per cell for aliskiren-treated cells and controls. The concentration of AngI in Medium-3 was determined by ELISA to calculate renin activity. Renin activity was divided by the cell number. Filled squares represent aliskiren-treated cells and open circles represent controls. Statistical differences were identified between aliskiren-treated cells and controls by a two-tailed unpaired Student's t-test with P<0.05 as the limit of significance. HUVEC, human umbilical vein endothelial cell; AngI, angiotensin I; (P)RR, (pro)renin receptor.

    Article Snippet: The concentration of purified prorenin was measured using a human prorenin enzyme-linked immunosorbent assay (ELISA) kit (cat no. IHPRENKT-NP; Innovative Research, Inc., Novi, MI, USA).

    Techniques: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Two Tailed Test

    Effect of aliskiren on the level of s(P)RR released from HUVECs. (A) Concentration of released s(P)RR. The concentration of s(P)RR in Medium-1 was determined by sandwich ELISA. (B) s(P)RR concentration per HUVEC cell. s(P)RR concentration was divided by the cell number. Filled squares represent aliskiren-treated cells and open circles represent controls. Statistical differences were identified between aliskiren-treated cells and controls by a two-tailed unpaired Student's t-test with P<0.05 as the limit of significance. s(P)RR, soluble (pro)renin receptor; HUVEC, human umbilical vein endothelial cell.

    Journal: Biomedical Reports

    Article Title: Aliskiren reduces the release of soluble (pro)renin receptor from human umbilical vein endothelial cells

    doi: 10.3892/br.2018.1124

    Figure Lengend Snippet: Effect of aliskiren on the level of s(P)RR released from HUVECs. (A) Concentration of released s(P)RR. The concentration of s(P)RR in Medium-1 was determined by sandwich ELISA. (B) s(P)RR concentration per HUVEC cell. s(P)RR concentration was divided by the cell number. Filled squares represent aliskiren-treated cells and open circles represent controls. Statistical differences were identified between aliskiren-treated cells and controls by a two-tailed unpaired Student's t-test with P<0.05 as the limit of significance. s(P)RR, soluble (pro)renin receptor; HUVEC, human umbilical vein endothelial cell.

    Article Snippet: The concentration of purified prorenin was measured using a human prorenin enzyme-linked immunosorbent assay (ELISA) kit (cat no. IHPRENKT-NP; Innovative Research, Inc., Novi, MI, USA).

    Techniques: Concentration Assay, Sandwich ELISA, Two Tailed Test

    Effect of aliskiren on the cell-surface expression of full-length (P)RR. (A) Percentage of prorenin bound to the HUVEC membrane. The concentration of unbound prorenin in Medium-2 was determined by ELISA, and the bound prorenin was determined as described in the Materials and methods section. (B) Percentage of bound prorenin per HUVEC. The percentage of bound prorenin was divided by the cell number. (C) Renin activity per cell for aliskiren-treated cells and controls. The concentration of AngI in Medium-3 was determined by ELISA to calculate renin activity. Renin activity was divided by the cell number. Filled squares represent aliskiren-treated cells and open circles represent controls. Statistical differences were identified between aliskiren-treated cells and controls by a two-tailed unpaired Student's t-test with P<0.05 as the limit of significance. HUVEC, human umbilical vein endothelial cell; AngI, angiotensin I; (P)RR, (pro)renin receptor.

    Journal: Biomedical Reports

    Article Title: Aliskiren reduces the release of soluble (pro)renin receptor from human umbilical vein endothelial cells

    doi: 10.3892/br.2018.1124

    Figure Lengend Snippet: Effect of aliskiren on the cell-surface expression of full-length (P)RR. (A) Percentage of prorenin bound to the HUVEC membrane. The concentration of unbound prorenin in Medium-2 was determined by ELISA, and the bound prorenin was determined as described in the Materials and methods section. (B) Percentage of bound prorenin per HUVEC. The percentage of bound prorenin was divided by the cell number. (C) Renin activity per cell for aliskiren-treated cells and controls. The concentration of AngI in Medium-3 was determined by ELISA to calculate renin activity. Renin activity was divided by the cell number. Filled squares represent aliskiren-treated cells and open circles represent controls. Statistical differences were identified between aliskiren-treated cells and controls by a two-tailed unpaired Student's t-test with P<0.05 as the limit of significance. HUVEC, human umbilical vein endothelial cell; AngI, angiotensin I; (P)RR, (pro)renin receptor.

    Article Snippet: The concentration of purified prorenin was measured using a human prorenin enzyme-linked immunosorbent assay (ELISA) kit (cat no. IHPRENKT-NP; Innovative Research, Inc., Novi, MI, USA).

    Techniques: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Two Tailed Test

    Effect of aliskiren on the level of s(P)RR released from HUVECs. (A) Concentration of released s(P)RR. The concentration of s(P)RR in Medium-1 was determined by sandwich ELISA. (B) s(P)RR concentration per HUVEC cell. s(P)RR concentration was divided by the cell number. Filled squares represent aliskiren-treated cells and open circles represent controls. Statistical differences were identified between aliskiren-treated cells and controls by a two-tailed unpaired Student's t-test with P<0.05 as the limit of significance. s(P)RR, soluble (pro)renin receptor; HUVEC, human umbilical vein endothelial cell.

    Journal: Biomedical Reports

    Article Title: Aliskiren reduces the release of soluble (pro)renin receptor from human umbilical vein endothelial cells

    doi: 10.3892/br.2018.1124

    Figure Lengend Snippet: Effect of aliskiren on the level of s(P)RR released from HUVECs. (A) Concentration of released s(P)RR. The concentration of s(P)RR in Medium-1 was determined by sandwich ELISA. (B) s(P)RR concentration per HUVEC cell. s(P)RR concentration was divided by the cell number. Filled squares represent aliskiren-treated cells and open circles represent controls. Statistical differences were identified between aliskiren-treated cells and controls by a two-tailed unpaired Student's t-test with P<0.05 as the limit of significance. s(P)RR, soluble (pro)renin receptor; HUVEC, human umbilical vein endothelial cell.

    Article Snippet: The concentration of purified prorenin was measured using a human prorenin enzyme-linked immunosorbent assay (ELISA) kit (cat no. IHPRENKT-NP; Innovative Research, Inc., Novi, MI, USA).

    Techniques: Concentration Assay, Sandwich ELISA, Two Tailed Test